Journal: American journal of physiology. Heart and circulatory physiology

Article Title: Novel anti-inflammatory mechanisms of N -Acetyl-Ser-Asp-Lys-Pro in hypertension-induced target organ damage

doi: 10.1152/ajpheart.00305.2007

Figure Lengend Snippet: FACS analysis to compare expression of F4/80 by macrophages. A: surface expression of F4/80 by macrophages obtained by treating bone marrow stem cells (BMSC) with macrophage growth medium (MGM). B: isotype control for A. C: surface F4/80 expression in cells treated with MGM + N-acetyl-serylaspartyl-lysyl-proline (Ac-SDKP; 10 nM). D: isotype control for C. FL1-H, fluorescence intensity on the FL1, (FITC) channel; M1, marker used to define positive events.

Article Snippet: The collected bone marrow cells were pooled, washed in ice-cold PBS, and resuspended in macrophage growth medium (MGM; BioVeris) with the following composition: human colony stimulating factors [granulocyte macrophage (GM)-CSF, granulocyte (G)-CSF, and macrophage colony-stimulating factor (M-CSF)] and cytokines in Iscove’s modified Dulbecco’s medium (IMDM; HEPES buffered) with 5% (vol/vol) FBS (heat inactivated) and kanamycin sulfate.

Techniques: Expressing, Control, Fluorescence, Marker

Journal: American journal of physiology. Heart and circulatory physiology

Article Title: Novel anti-inflammatory mechanisms of N -Acetyl-Ser-Asp-Lys-Pro in hypertension-induced target organ damage

doi: 10.1152/ajpheart.00305.2007

Figure Lengend Snippet: Measurement of macrophage migration in the Boyden chamber. Cells were plated on the upper surface of the Boyden chamber and a chemoattractant [galectin-3 or macrophage colony-stimulating factor (M-CSF)] with and without Ac-SDKP was added to the lower chamber. A: effects of Ac-SDKP on macrophage migration stimulated with galectin-3. B: effects of Ac-SDKP on macrophage migration stimulated with M-CSF. Ac-SDKP inhibited both galectin-3- and M-CSF-dependent macrophage migration in vitro. A.U., arbitrary units; n = 4.

Article Snippet: The collected bone marrow cells were pooled, washed in ice-cold PBS, and resuspended in macrophage growth medium (MGM; BioVeris) with the following composition: human colony stimulating factors [granulocyte macrophage (GM)-CSF, granulocyte (G)-CSF, and macrophage colony-stimulating factor (M-CSF)] and cytokines in Iscove’s modified Dulbecco’s medium (IMDM; HEPES buffered) with 5% (vol/vol) FBS (heat inactivated) and kanamycin sulfate.

Techniques: Migration, In Vitro

Journal: American journal of physiology. Heart and circulatory physiology

Article Title: Novel anti-inflammatory mechanisms of N -Acetyl-Ser-Asp-Lys-Pro in hypertension-induced target organ damage

doi: 10.1152/ajpheart.00305.2007

Figure Lengend Snippet: Effect of Ac-SDKP on TNF-α release by cultured macrophages stimulated with LPS. Macrophages were grown in the 96-well culture plate; TNF-α levels were measured in the culture supernatant and were normalized to the viable cells present on the corresponding culture wells. Bacterial LPS increased the release of TNF-α by mature macrophages, which was significantly reduced by Ac-SDKP; n = 6.

Article Snippet: The collected bone marrow cells were pooled, washed in ice-cold PBS, and resuspended in macrophage growth medium (MGM; BioVeris) with the following composition: human colony stimulating factors [granulocyte macrophage (GM)-CSF, granulocyte (G)-CSF, and macrophage colony-stimulating factor (M-CSF)] and cytokines in Iscove’s modified Dulbecco’s medium (IMDM; HEPES buffered) with 5% (vol/vol) FBS (heat inactivated) and kanamycin sulfate.

Techniques: Cell Culture